e coli cft073 strain Search Results


e coli cft073 genomic dna  (ATCC)
93
ATCC e coli cft073 genomic dna
E Coli Cft073 Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli cft073 genomic dna - by Bioz Stars, 2026-03
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cft073  (ATCC)
96
ATCC cft073
Colonization by the parent strain <t>(CFT073)</t> and ompX mutant in the bladders and kidneys of mice with UTIs. The female mice (n = 3 for each group) were infected with the parent strain or ompX mutant. At 48 h postinfection, cell numbers of bacteria isolated from the bladder and kidneys were determined as CFU. We repeated experiments independently three times, but one mouse infected with the parent strain died within 48 h of infection. Each data point represents a sample from an individual mouse (n = 8 for the parent strain and n = 9 for the ompX mutant). Horizontal bars show median values. *, P < 0.05 relative to the value for the parent strain.
Cft073, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cft073 - by Bioz Stars, 2026-03
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uropathogenic escherichia coli strain cft073  (Miethke GmbH)
90
Miethke GmbH uropathogenic escherichia coli strain cft073
Colonization by the parent strain <t>(CFT073)</t> and ompX mutant in the bladders and kidneys of mice with UTIs. The female mice (n = 3 for each group) were infected with the parent strain or ompX mutant. At 48 h postinfection, cell numbers of bacteria isolated from the bladder and kidneys were determined as CFU. We repeated experiments independently three times, but one mouse infected with the parent strain died within 48 h of infection. Each data point represents a sample from an individual mouse (n = 8 for the parent strain and n = 9 for the ompX mutant). Horizontal bars show median values. *, P < 0.05 relative to the value for the parent strain.
Uropathogenic Escherichia Coli Strain Cft073, supplied by Miethke GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli reference strains expec cft073  (Inserm Transfert)
90
Inserm Transfert e. coli reference strains expec cft073
HEp-2 invasiveness of the 36 <t> AIEC </t> isolated from patients with CD (CD1-4) and from non-IBD controls (Control 1-4)
E. Coli Reference Strains Expec Cft073, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
e. coli reference strains expec cft073 - by Bioz Stars, 2026-03
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Image Search Results


Colonization by the parent strain (CFT073) and ompX mutant in the bladders and kidneys of mice with UTIs. The female mice (n = 3 for each group) were infected with the parent strain or ompX mutant. At 48 h postinfection, cell numbers of bacteria isolated from the bladder and kidneys were determined as CFU. We repeated experiments independently three times, but one mouse infected with the parent strain died within 48 h of infection. Each data point represents a sample from an individual mouse (n = 8 for the parent strain and n = 9 for the ompX mutant). Horizontal bars show median values. *, P < 0.05 relative to the value for the parent strain.

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Colonization by the parent strain (CFT073) and ompX mutant in the bladders and kidneys of mice with UTIs. The female mice (n = 3 for each group) were infected with the parent strain or ompX mutant. At 48 h postinfection, cell numbers of bacteria isolated from the bladder and kidneys were determined as CFU. We repeated experiments independently three times, but one mouse infected with the parent strain died within 48 h of infection. Each data point represents a sample from an individual mouse (n = 8 for the parent strain and n = 9 for the ompX mutant). Horizontal bars show median values. *, P < 0.05 relative to the value for the parent strain.

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Mutagenesis, Infection, Bacteria, Isolation

Adhesion to and internalization in kidney epithelial cells (HTB-44) of the parent strain (CFT073) and the ompX mutant (A and B) or the parent strain and the ompX mutant carrying pTrc99K (empty vector) or pTrc99KompX (ompX expression plasmid) (C). Values are percent CFU of adhered/internalized (A) and internalized (B and C) bacteria relative to total bacterial cell numbers. Data are means from three independent experiments; error bars indicate standard deviations. *, P < 0.05 relative to values for CFT073 (A and B) or CFT073/pTrc99K (C).

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Adhesion to and internalization in kidney epithelial cells (HTB-44) of the parent strain (CFT073) and the ompX mutant (A and B) or the parent strain and the ompX mutant carrying pTrc99K (empty vector) or pTrc99KompX (ompX expression plasmid) (C). Values are percent CFU of adhered/internalized (A) and internalized (B and C) bacteria relative to total bacterial cell numbers. Data are means from three independent experiments; error bars indicate standard deviations. *, P < 0.05 relative to values for CFT073 (A and B) or CFT073/pTrc99K (C).

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Mutagenesis, Plasmid Preparation, Expressing, Bacteria

Aggregation within kidney epithelial cells (HTB-44) for the parent strain and the ompX mutant or the parent strain and the ompX mutant carrying pTH18kr (empty vector) or pTH18krompX (ompX expression plasmid). Bacteria carrying a green fluorescence protein (GFP) expression plasmid, pTurboGFP-B, and HTB-44 cells stained with rhodamine-phalloidin were imaged with green and red fluorescence, respectively, using a 100× objective. Images were taken from above (A), and cross-sectional images (B) correspond to the white boxes in panel A. The experiment was repeated twice, and similar results were obtained. (C) Aggregated bacteria within HTB-44 cells were quantified by representing levels of colonized bacteria as areas (in square pixels) of GFP. Microscopy data are means from three fields of view, and error bars indicate standard deviations. **, P < 0.01 relative to the value for the parent strain CFT073.

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Aggregation within kidney epithelial cells (HTB-44) for the parent strain and the ompX mutant or the parent strain and the ompX mutant carrying pTH18kr (empty vector) or pTH18krompX (ompX expression plasmid). Bacteria carrying a green fluorescence protein (GFP) expression plasmid, pTurboGFP-B, and HTB-44 cells stained with rhodamine-phalloidin were imaged with green and red fluorescence, respectively, using a 100× objective. Images were taken from above (A), and cross-sectional images (B) correspond to the white boxes in panel A. The experiment was repeated twice, and similar results were obtained. (C) Aggregated bacteria within HTB-44 cells were quantified by representing levels of colonized bacteria as areas (in square pixels) of GFP. Microscopy data are means from three fields of view, and error bars indicate standard deviations. **, P < 0.01 relative to the value for the parent strain CFT073.

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Mutagenesis, Plasmid Preparation, Expressing, Bacteria, Fluorescence, Staining, Microscopy

Motilities and flagellar production for the parent strain (CFT073) and the ompX mutant or the parent strain and the ompX mutant carrying pTrc99K (empty vector) or pTrc99KompX (ompX expression plasmid). (A) Bacterial migration on LB medium containing 0.3% agar. (B) Diameters reflecting bacterial migration on the agar. Data are means from three independent experiments; error bars indicate standard deviations. (C) Flagella and bacterial cells were stained with Victoria blue/tannic acid were pictured using a 100× objective. (D) Ratios of bacteria observed with flagella to ∼120 to 150 randomly selected bacteria on microscopy, presented as percentages. Data are means, and error bars indicate standard deviations. **, P < 0.01 relative to the value for CFT073.

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Motilities and flagellar production for the parent strain (CFT073) and the ompX mutant or the parent strain and the ompX mutant carrying pTrc99K (empty vector) or pTrc99KompX (ompX expression plasmid). (A) Bacterial migration on LB medium containing 0.3% agar. (B) Diameters reflecting bacterial migration on the agar. Data are means from three independent experiments; error bars indicate standard deviations. (C) Flagella and bacterial cells were stained with Victoria blue/tannic acid were pictured using a 100× objective. (D) Ratios of bacteria observed with flagella to ∼120 to 150 randomly selected bacteria on microscopy, presented as percentages. Data are means, and error bars indicate standard deviations. **, P < 0.01 relative to the value for CFT073.

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Mutagenesis, Plasmid Preparation, Expressing, Migration, Staining, Bacteria, Microscopy

FliC expression in the ompX mutant and contribution of fliC to bacterial adhesion to and internalization within the kidney epithelial cells. (A) Western blots of cell lysates and secreted proteins from the parent strain (CFT073) and the ompX mutant containing a VSVG-tagged FliC expression plasmid (pTH18krfliC-VSVG) and pTrc99A (empty vector) or pTrc99AompX (ompX expression plasmid). Locations of molecular mass standards (in kilodaltons) are shown on the left. VSVG-tagged FliC was visualized by probing with a VSVG antibody. Adhesion to and internalization in kidney epithelial cells (HTB-44) of the parent strain, fliC mutant, and fliC/ompX double mutant (B and C) or the parent strain and the fliC mutant carrying pTrc99A (empty vector) or pTrc99AfliC (fliC expression plasmid) (D). Values are percent CFU of adhered/internalized (B) and internalized (C and D) bacteria relative to total bacterial cell numbers. Data are means from three independent experiments; error bars indicate standard deviations. *, P < 0.05 relative to CFT073 (B and C) or CFT073/pTrc99A (D).

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: FliC expression in the ompX mutant and contribution of fliC to bacterial adhesion to and internalization within the kidney epithelial cells. (A) Western blots of cell lysates and secreted proteins from the parent strain (CFT073) and the ompX mutant containing a VSVG-tagged FliC expression plasmid (pTH18krfliC-VSVG) and pTrc99A (empty vector) or pTrc99AompX (ompX expression plasmid). Locations of molecular mass standards (in kilodaltons) are shown on the left. VSVG-tagged FliC was visualized by probing with a VSVG antibody. Adhesion to and internalization in kidney epithelial cells (HTB-44) of the parent strain, fliC mutant, and fliC/ompX double mutant (B and C) or the parent strain and the fliC mutant carrying pTrc99A (empty vector) or pTrc99AfliC (fliC expression plasmid) (D). Values are percent CFU of adhered/internalized (B) and internalized (C and D) bacteria relative to total bacterial cell numbers. Data are means from three independent experiments; error bars indicate standard deviations. *, P < 0.05 relative to CFT073 (B and C) or CFT073/pTrc99A (D).

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Expressing, Mutagenesis, Western Blot, Plasmid Preparation, Bacteria

Aggregation within kidney epithelial cells (HTB-44) for the parent strain and the fliC mutant or the parent strain and the fliC mutant carrying pTH18kr (empty vector) or pTH18krfliC-VSVG (fliC expression plasmid). Bacteria carrying a GFP expression plasmid, pTurboGFP-B, and HTB-44 cells stained with rhodamine-phalloidin were imaged with green and red fluorescence, respectively, using a 100× objective. Images were taken from above (A), and cross-sectional images (B) correspond to the white boxes in panel A. The experiment was repeated twice, and similar results were obtained. (C) Aggregated bacteria within HTB-44 cells were quantified by determining levels of colonized bacteria as areas (in square pixels) of GFP. Microscopy data are means from three fields of view, and error bars indicate standard deviations. *, P < 0.05, and **, P < 0.01, relative to CFT073.

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Aggregation within kidney epithelial cells (HTB-44) for the parent strain and the fliC mutant or the parent strain and the fliC mutant carrying pTH18kr (empty vector) or pTH18krfliC-VSVG (fliC expression plasmid). Bacteria carrying a GFP expression plasmid, pTurboGFP-B, and HTB-44 cells stained with rhodamine-phalloidin were imaged with green and red fluorescence, respectively, using a 100× objective. Images were taken from above (A), and cross-sectional images (B) correspond to the white boxes in panel A. The experiment was repeated twice, and similar results were obtained. (C) Aggregated bacteria within HTB-44 cells were quantified by determining levels of colonized bacteria as areas (in square pixels) of GFP. Microscopy data are means from three fields of view, and error bars indicate standard deviations. *, P < 0.05, and **, P < 0.01, relative to CFT073.

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Mutagenesis, Plasmid Preparation, Expressing, Bacteria, Staining, Fluorescence, Microscopy

Transcript levels and promoter activities of flagellum-related and fimbrial genes in the parent strain (CFT073) and the ompX mutant. (A) Transcript levels were determined relative to that of rpoD. Data are means for two biological replicates; error bars indicate the ranges. (B) β-Galactosidase activities corresponding to flhD, fliA, and fliC promoter activities in the parent strain and the ompX mutant containing pNNflhD-P, pNNfliA-P, or pNNfliC-P, the lacZ reporter plasmid. Data are means from three independent experiments; error bars indicate standard deviations. **, P < 0.01 relative to CFT073.

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Transcript levels and promoter activities of flagellum-related and fimbrial genes in the parent strain (CFT073) and the ompX mutant. (A) Transcript levels were determined relative to that of rpoD. Data are means for two biological replicates; error bars indicate the ranges. (B) β-Galactosidase activities corresponding to flhD, fliA, and fliC promoter activities in the parent strain and the ompX mutant containing pNNflhD-P, pNNfliA-P, or pNNfliC-P, the lacZ reporter plasmid. Data are means from three independent experiments; error bars indicate standard deviations. **, P < 0.01 relative to CFT073.

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Mutagenesis, Plasmid Preparation

Strains and plasmids used in this study a

Journal: Infection and Immunity

Article Title: Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

doi: 10.1128/IAI.00721-20

Figure Lengend Snippet: Strains and plasmids used in this study a

Article Snippet: CFT073 , Parent strain (ATCC 700928) , ATCC 700928.

Techniques: Plasmid Preparation, Mutagenesis, Expressing

HEp-2 invasiveness of the 36 AIEC isolated from patients with CD (CD1-4) and from non-IBD controls (Control 1-4)

Journal: BMC Research Notes

Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

doi: 10.1186/1756-0500-7-748

Figure Lengend Snippet: HEp-2 invasiveness of the 36 AIEC isolated from patients with CD (CD1-4) and from non-IBD controls (Control 1-4)

Article Snippet: AIEC strain LF82 and E. coli reference strains EAEC 042, ETEC EDL 1493, DAEC C1845 and ExPEC CFT073 (provided by Prof. P. Escobar-Paramo; INSERM, Paris, France) were used to compare genotypic and phenotypic characteristics of clinical isolates.

Techniques: Isolation, Control

Thirty-six adherent/invasive E. coli isolates were able to persist and to replicate within J774 macrophages

Journal: BMC Research Notes

Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

doi: 10.1186/1756-0500-7-748

Figure Lengend Snippet: Thirty-six adherent/invasive E. coli isolates were able to persist and to replicate within J774 macrophages

Article Snippet: AIEC strain LF82 and E. coli reference strains EAEC 042, ETEC EDL 1493, DAEC C1845 and ExPEC CFT073 (provided by Prof. P. Escobar-Paramo; INSERM, Paris, France) were used to compare genotypic and phenotypic characteristics of clinical isolates.

Techniques: Control

Genetic variability among the 36 AIEC isolates from CD patients CD1, CD2 and CD3, and from non-IBD controls Control-1 and Control-2. Consensus UPGMA dendrogram, generated on Dice coefficients of Xba I PFGE and RAPD-PCR combined profiles (similarity cut-off level 95%) identified 27 genetic variants (22 from CD patients and 5 for controls). Genotypes of AIEC strains isolated from the same patient tended to cluster.

Journal: BMC Research Notes

Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

doi: 10.1186/1756-0500-7-748

Figure Lengend Snippet: Genetic variability among the 36 AIEC isolates from CD patients CD1, CD2 and CD3, and from non-IBD controls Control-1 and Control-2. Consensus UPGMA dendrogram, generated on Dice coefficients of Xba I PFGE and RAPD-PCR combined profiles (similarity cut-off level 95%) identified 27 genetic variants (22 from CD patients and 5 for controls). Genotypes of AIEC strains isolated from the same patient tended to cluster.

Article Snippet: AIEC strain LF82 and E. coli reference strains EAEC 042, ETEC EDL 1493, DAEC C1845 and ExPEC CFT073 (provided by Prof. P. Escobar-Paramo; INSERM, Paris, France) were used to compare genotypic and phenotypic characteristics of clinical isolates.

Techniques: Control, Generated, Isolation

Similarity analysis between AIEC genotypes isolated from CD patients and non-IBD controls. Percentage values were based on Dice similarity index. A P value ≤0.05 was considered statistically significant.

Journal: BMC Research Notes

Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

doi: 10.1186/1756-0500-7-748

Figure Lengend Snippet: Similarity analysis between AIEC genotypes isolated from CD patients and non-IBD controls. Percentage values were based on Dice similarity index. A P value ≤0.05 was considered statistically significant.

Article Snippet: AIEC strain LF82 and E. coli reference strains EAEC 042, ETEC EDL 1493, DAEC C1845 and ExPEC CFT073 (provided by Prof. P. Escobar-Paramo; INSERM, Paris, France) were used to compare genotypic and phenotypic characteristics of clinical isolates.

Techniques: Isolation

Adhesion phenotypes assay. Phase contrast fields showing Hep-2 and Caco-2 cell monolayers infected with two representative AIEC strains isolated in this work (PP45 and DF6) and with reference AIEC LF82 strain. All 27 AIEC strains showed an aggregative (AA) patterns of adherence. Magnification, 1000 X.

Journal: BMC Research Notes

Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

doi: 10.1186/1756-0500-7-748

Figure Lengend Snippet: Adhesion phenotypes assay. Phase contrast fields showing Hep-2 and Caco-2 cell monolayers infected with two representative AIEC strains isolated in this work (PP45 and DF6) and with reference AIEC LF82 strain. All 27 AIEC strains showed an aggregative (AA) patterns of adherence. Magnification, 1000 X.

Article Snippet: AIEC strain LF82 and E. coli reference strains EAEC 042, ETEC EDL 1493, DAEC C1845 and ExPEC CFT073 (provided by Prof. P. Escobar-Paramo; INSERM, Paris, France) were used to compare genotypic and phenotypic characteristics of clinical isolates.

Techniques: Infection, Isolation

Prevalence of virulence genes, identified for each strain by PCR, in the 22 AIEC strains in CD and in the 5 non-IBD pediatric patients

Journal: BMC Research Notes

Article Title: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

doi: 10.1186/1756-0500-7-748

Figure Lengend Snippet: Prevalence of virulence genes, identified for each strain by PCR, in the 22 AIEC strains in CD and in the 5 non-IBD pediatric patients

Article Snippet: AIEC strain LF82 and E. coli reference strains EAEC 042, ETEC EDL 1493, DAEC C1845 and ExPEC CFT073 (provided by Prof. P. Escobar-Paramo; INSERM, Paris, France) were used to compare genotypic and phenotypic characteristics of clinical isolates.

Techniques: Sequencing, Plasmid Preparation